Pfu DNA Polymerase

Pfu DNA polymerase is a high-fidelity, thermostable DNA polymerase with 5´→3´ DNA polymerase and 3´→5´ exonuclease (proofreading) activities. It is approximately 90kDa and isolated from Pyrococcus furiosus. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an increased fidelity of DNA synthesis (six-fold) compared to Taq polymerase. Due to its proofreading activity, Pfu DNA Polymerase is recommended for use in PCR reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended which can be further used in cloning. The product contains an optional 5X GC Enhancer which increases the amplification of GC rich templates up to 84%.

 

Product Specifications:

Catalog Number: ZXB-06-144

Concentration: 5 U/μL

Fidelity: 6.0 times higher fidelity than Taq DNA Polymerase

Purity: >98%

Unit definition: One unit is defined as the amount of enzyme that catalyzed the incorporation of 10 nmol of dNTPs into acid-insoluble form in 30 minutes at 72°C.

Storage: -20°C

 

SDS  |  CoA

 

179,00 €

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