Agarose beads consist of small spherical particles of agarose which are different in diameters and concentrations and are widely used for size exclusion or affinity chromatography. Standard, cross-linked and highly cross-linked agarose beads (without ligand linking) are used in Gel Filtration Chromatography (or size Exclusion Chromatography), while, for affinity chromatography, ligands such as nitrilotriacetic acid (NTA) or iminodiacetic acid (IDA) are covalently linked to the agarose bead polymer which can be further used for separation of tagged proteins from other proteins. For decades, Agarose beads have been used for protein purification. Physical characteristics of agarose beads significantly vary between different kinds of beads and result in their different applications. Standard agarose beads (not cross-linked) are used for separation techniques whereby gravity force runs the samples through the agarose matrix. However, for high-pressure separation techniques, highly cross-linked and cross-linked beads must be chosen. Highly cross-linked and cross-linked beads are physically and chemically more stable than the standard agarose beads, hence, the higher the level of cross-linking is the more stable the matrix are. To produce Highly cross-linked and cross-linked beads, agarose beads must be treated with a particular reagent.
Protein G Agarose Beads (4%) Highly Cross-linked consist of 4 percent highly cross-linked agarose beads with size of 50-150 µm which covalently bound to rProtein G. This resin provides a very stable bond that greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. It works well in High Flow conditions and used to purify classes, subclasses and fragments of immunoglobulins from cell culture media and other biological fluids.
Catalog Number: ZXB-06-158
Bead Geometry & Size: Spherical ~50-150 µm
Agarose %: 4%
Coupling Method: Binding by reductive amination
Binding Capacity: ~20 mg human IgG/ ml resin
Antimicrobial Agent: 20% Ethanol
Storage Temperature: 4˚C
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